Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human (U2OS) mouse (MEF) rat (RIE1))
Specification
Human (U2OS) mouse (MEF) rat (RIE1)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-100 [PBS]
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Donkey Alexa Fluor 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
投稿 Apr 10 2008