Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human and mouse (pancreas), rat (testis))
Specification
human and mouse (pancreas), rat (testis)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Stains well though would recommend a user-specfic dilution in the range 1/100 - 1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
投稿 Apr 08 2008