Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDAMB435S)
Specification
MDAMB435S
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 30 minute(s) · Diluent: 3% BSA/PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 555 Anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000
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投稿 Feb 28 2013