Anti-PPAR delta 抗体 (ab23673)
Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PPAR delta antibody
PPAR delta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPAR delta -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Chicken -
免疫原
Synthetic peptide corresponding to Human PPAR delta aa 1-100 (N terminal).
Database link: Q03181 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23673の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 4 µg/ml.
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WB | (6) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa. Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 4 µg/ml. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa. Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa. |
ターゲット情報
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機能
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
組織特異性
Ubiquitous with maximal levels in placenta and skeletal muscle. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 395479 Chicken
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
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別名
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
画像
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Anti-PPAR delta antibody (ab23673) at 1/1000 dilution + Rat brain tissue lysate at 100 µg
Secondary
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.
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All lanes : Anti-PPAR delta antibody (ab23673)
Lane 1 : Human cerebral cortex (30ug)
Lane 2 : Murine liver (30ug)
Predicted band size: 50 kDa
ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta (~40kDa) is also detected in certain murine tissues.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (44)
ab23673 は 44 報の論文で使用されています。
- Valdivia AO & Bhattacharya SK Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function. eNeuro 9:N/A (2022). PubMed: 35027445
- Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
- Brailey PM et al. CD1d-dependent rewiring of lipid metabolism in macrophages regulates innate immune responses. Nat Commun 13:6723 (2022). PubMed: 36344546
- Ortega MA et al. Defective expression of the peroxisome regulators PPARα receptors and lysogenesis with increased cellular senescence in the venous wall of chronic venous disorder. Histol Histopathol 36:547-558 (2021). PubMed: 33645625
- Wang YL et al. Laminar shear stress upregulates the expression of PPARs in vascular endothelial cells under high free fatty acid-induced stress. Exp Ther Med 21:438 (2021). PubMed: 33747175