Anti-PGC1 alpha 抗体 - N-terminal (ab191838)
Key features and details
- Rabbit polyclonal to PGC1 alpha - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PGC1 alpha antibody - N-terminal
PGC1 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PGC1 alpha - N-terminal -
由来種
Rabbit -
特異性
At least three isoforms of PGC1 alpha are known to exist; this antibody will detect all three isoforms. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Pig -
免疫原
Synthetic peptide within Human PGC1 alpha (N terminal). The exact sequence is proprietary. The immunogen is located within amino acids 200 - 250 of PGC1 alpha and consists of a 17 amino acid peptide.
Database link: Q9UBK2 -
ポジティブ・コントロール
- WB: Human heart and lung lysate. Rat kidney lysate. Mouse skin lysate. Daudi and K562 whole cell lysates. IHC-P: Rat heart and kidney tissue. Human pancreas tissue. ICC/IF: HeLa cells.
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特記事項
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191838の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 91 kDa.
Blocking in 5% non-fat milk in TBS at 4 overnight. ab191838 incubation at room temperature for 1 hour. |
|
IHC-P | (2) |
1/500.
|
ICC/IF |
1/500.
|
特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 91 kDa. Blocking in 5% non-fat milk in TBS at 4 overnight. ab191838 incubation at room temperature for 1 hour. |
IHC-P
1/500. |
ICC/IF
1/500. |
ターゲット情報
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機能
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. -
組織特異性
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. -
配列類似性
Contains 1 RRM (RNA recognition motif) domain. -
翻訳後修飾
Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. -
細胞内局在
Cytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body. - Information by UniProt
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参照データベース
- Entrez Gene: 338446 Cow
- Entrez Gene: 10891 Human
- Entrez Gene: 19017 Mouse
- Entrez Gene: 497232 Pig
- Entrez Gene: 83516 Rat
- Omim: 604517 Human
- SwissProt: Q865B7 Cow
- SwissProt: Q9UBK2 Human
see all -
別名
- L PGC 1alpha antibody
- LEM6 antibody
- Ligand effect modulator 6 antibody
see all
画像
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Anti-PGC1 alpha antibody - N-terminal (ab191838) at 2 µg/ml + Rat kidney lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/10000 dilution
Predicted band size: 91 kDaab191838 was incubated for 1 hour at room temperature in 5% NFDM/TBST.
Exposure: 5 min
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Immunohistochemistry (Formalin-fixed paraffin embedded sections) of rat kidney tissue labeling PGC1 alpha with Anti-PGC1 alpha antibody - N-terminal (ab191838) at a 1/500 dilution. ABC HRP Kit (Rabbit IgG) was used as a secondary antibody at 1/200 dilution.
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Immunofluorescent analysis of Hela cells (4% PFA, RT, 15min, 0.1% Triton X-100 in PBS, RT, 4min) labeling PGC1 alpha with ab191838 at 1/500 dilution. Rabbit IgG antibody (Alexa Fluor 488) was used as a secondary antibody at 1:2000.
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Anti-PGC1 alpha antibody - N-terminal (ab191838) at 2 µg/ml + Human lung lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/10000 dilution
Predicted band size: 91 kDaab191838 was incubated for 1 hour at room temperature in 5% NFDM/TBST.
Exposure: 5 min
-
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human pancreas tissue labeling PGC1 alpha with Anti-PGC1 alpha antibody - N-terminal (ab191838) at a 1/500 dilution. ABC HRP Kit (Rabbit IgG) was used as a secondary antibody at 1/200 dilution.
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Anti-PGC1 alpha antibody - N-terminal (ab191838) at 2 µg/ml + Mouse skin lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP at 1/10000 dilution
Predicted band size: 91 kDaab191838 was incubated for 1 hour at room temperature in 5% NFDM/TBST.
Exposure: 5 min
-
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human pancreas tissue labeling PGC1 alpha with Anti-PGC1 alpha antibody - N-terminal (ab191838) at a 1/500 dilution. ABC HRP Kit (Rabbit IgG) was used as a secondary antibody at 1/200 dilution.
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All lanes : Anti-PGC1 alpha antibody - N-terminal (ab191838) at 2 µg/ml
Lane 1 : Duadi whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP at 1/10000 dilution
Predicted band size: 91 kDaab191838 was incubated for 1 hour at room temperature in 5% NFDM/TBST.
Exposure: 1 min
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (44)
ab191838 は 44 報の論文で使用されています。
- Wang J et al. Glutamine, glutamate, and aspartate differently modulate energy homeostasis of small intestine under normal or low energy status in piglets. Anim Nutr 8:216-226 (2022). PubMed: 34977390
- Chen JW et al. mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis. Front Cell Neurosci 16:803718 (2022). PubMed: 35210991
- Zhang Z et al. Neuroprotective Effects of a Cholecystokinin Analogue in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine Parkinson's Disease Mouse Model. Front Neurosci 16:814430 (2022). PubMed: 35368248
- Xu Z et al. The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex. Am J Cancer Res 12:1372-1392 (2022). PubMed: 35411229
- Gao Z et al. Hypoxic mesenchymal stem cell-derived extracellular vesicles ameliorate renal fibrosis after ischemia-reperfusion injure by restoring CPT1A mediated fatty acid oxidation. Stem Cell Res Ther 13:191 (2022). PubMed: 35526054