The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
Use at an assay dependent concentration.
Is unsuitable for Flow Cyt,ICC/IF or IP.
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Appears to be expressed ubiquitously.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. Contains 1 PH domain. Contains 1 protein kinase domain.
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
3 phosphoinositide dependent protein kinase 1 antibody
3-phosphoinositide-dependent protein kinase 1 antibody
hPDK 1 antibody
PkB kinase antibody
PkB kinase like gene 1 antibody
PkB like 1 antibody
Protein kinase antibody
Western blot - Anti-PDPK1 antibody [EP569Y] (ab52893)
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: PDPK1 knockout HAP1 cell lysate (20 µg) Lane 3: HEK293 cell lysate (20 µg) Lane 4: MCF7 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab52893 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab52893 was shown to recognize PDPK1 when PDPK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PDPK1 knockout samples were subjected to SDS-PAGE. ab52893 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.