Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Chinese Hamster Ovary)
Permeabilization
Yes - 0,1 % TritonX-100 for 5 min @ RT
Specification
Chinese Hamster Ovary
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 3 % BSA/0.1 % Tween20/PBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor(R)488-conjugated AffiniPure Donkey Ant
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
We would like to thank the researcher for submitting an AbReview and the honest feedback. The antibody is validated in human and hamster cells and we resolved this issue in accordance with our AbPromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 20 2016