Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 0.5 % BSA/PBS
Secondary antibody
Name
Non-Abcam antibody was used: Dako REAL™ HRP/DAB+, Rabbit/Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Nuclei staining with Haematoxillin (blue) and PDGF receptor alpha staining with DAB (brown). We found membranouse staining of glia cells within the white matter of the mouse brain.
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投稿 Nov 16 2010