Application
Western blot
Sample
Mouse Tissue lysate - other (Liver, cytosol)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
Liver, cytosol
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 1% BSA
Concentration
1 µg/ml
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: ~69 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Werner Kovacs
Verified customer
投稿 Sep 30 2010