The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 mg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
Contains 3 KH domains.
Phosphorylated; lowers poly(rC)-binding activity.
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
ICC/IF image of ab74793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74793, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab74793 at 5µg/ml staining PCBP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue).
Western blot - Anti-PCBP1 antibody (ab74793)Image courtesy of an anonymous Abreview.
All lanes : Anti-PCBP1 antibody (ab74793) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells Lane 2 : Whole cell lysate prepared from F9 cells Lane 3 : Whole cell lysate prepared from GC1 cells Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 30 µg per lane.
Secondary All lanes : HRP conjugated pig anti-rabbit polyclonal
Emde A et al. Dysregulated miRNA biogenesis downstream of cellular stress and ALS-causing mutations: a new mechanism for ALS. EMBO J34:2633-51 (2015).
Read more (PubMed: 26330466) »
Ho JJ et al. Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs. Mol Cell Biol33:2029-46 (2013).
Read more (PubMed: 23478261) »