Application
Western blot
Sample
Human Cell lysate - whole cell (Human Bone Marrow Stromal Cells (HS-5))
Loading amount
5 µg
Specification
Human Bone Marrow Stromal Cells (HS-5)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
1x Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1x Casein
Secondary antibody
Name
Non-Abcam antibody was used: Anti-goat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
Supersignal West Dura
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 45 kDa Non-specific: 48 kDa
Additional data
Additional Notes
A double band was detected, with a specific band at 45kD and a slightly larger one at 48kD.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 23 2009