保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
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精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab31280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 1.5 - 3 µg/ml.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||Use a concentration of 4 - 6 µg/ml.|
機能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
組織特異性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
関連疾患Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
配列類似性Belongs to the serpin family.
翻訳後修飾Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
- Information by UniProt
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
Anti-PAI1 antibody (ab31280) at 0.1 µg/ml + Human Liver lysate at 35 µg
Predicted band size: 45 kDa
Observed band size: 45 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
ab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.
ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
ab31280 (1.5ug/ml) used as the reporter with a capture rabbit antibody (2.5ug/ml).
This product has been referenced in:
- Tsuge M et al. SK-216, a Novel Inhibitor of Plasminogen Activator Inhibitor-1, Suppresses Lung Metastasis of Human Osteosarcoma. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29510576) »
- Hirahata M et al. PAI-1, a target gene of miR-143, regulates invasion and metastasis by upregulating MMP-13 expression of human osteosarcoma. Cancer Med 5:892-902 (2016). WB . Read more (PubMed: 26817521) »