APC Anti-Nrf2 抗体 [EP1808Y] (ab223927)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EP1808Y] to Nrf2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
製品の概要
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製品名
APC Anti-Nrf2 antibody [EP1808Y]
Nrf2 一次抗体 製品一覧 -
製品の詳細
APC Rabbit monoclonal [EP1808Y] to Nrf2 -
由来種
Rabbit -
標識
APC. Ex: 645nm, Em: 660nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1808Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Nrf2 antibody [EP1808Y] - BSA and Azide free (ab180845)
- Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)
- Alexa Fluor® 647 Anti-Nrf2 antibody [EP1808Y] (ab194985)
- HRP Anti-Nrf2 antibody [EP1808Y] (ab194986)
- Alexa Fluor® 594 Anti-Nrf2 antibody [EP1808Y] (ab206890)
- PE Anti-Nrf2 antibody [EP1808Y] (ab223926)
- Anti-Nrf2 antibody [EP1808Y] (ab62352)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab223927の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
特記事項 |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
ターゲット情報
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機能
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. -
組織特異性
Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. -
配列類似性
Belongs to the bZIP family. CNC subfamily.
Contains 1 bZIP domain. -
ドメイン
Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus. -
翻訳後修飾
Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. - Information by UniProt
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参照データベース
- Entrez Gene: 4780 Human
- Omim: 600492 Human
- SwissProt: Q16236 Human
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別名
- erythroid derived 2 antibody
- HEBP1 antibody
- like 2 antibody
see all
画像
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Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab223927 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Triton X-100 for 15 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223927, 1/500 dilution) for 30 minutes at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 minutes)/permeabilized with 0.1% PBS-Triton X-100 for 15 minutes used under the same conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab223927 は 1 報の論文で使用されています。
- Niu LT et al. Targeting IFN-?-inducible lysosomal thiol reductase overcomes chemoresistance in AML through regulating the ROS-mediated mitochondrial damage. Transl Oncol 14:101159 (2021). PubMed: 34252711