Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS cells)
Permeabilization
Yes - Tween-20
Specification
U2OS cells
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/250
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG FITC Conjugated Ab
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 28 2017