Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Alexa647-conjugated goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/1000
Detection
Detection method
fluorescence scanning
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 32 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 26 2012