Application
Other - Do not use
Review text: Prior to using for fluoresecent confocal microscopy ab93152 (FITC-uPA) was tested for fibrinolytic activity by measuring clot lysis using a turbidity assay. Clot lysis was observed with 100 pM ab93152 (50 % lysis time = 130 min) prior to aliquoting and freezing as instructed in the data sheet. However, after freeze thawing activity was reduced (50 % lysis time = 248 min)
For observation of lysis by fluorescent confocal microscopy 75 nM ab93152 was used. No signal for the FITC uPA could be detected.
Sample: Human Recombinant protein
Primary antibody (in addition to 'Urokinase protein (FITC) (Human)')
Primary antibody: None used
Secondary antibody
Secondary antibody: None used
Additional data
Abcam response: Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Claire Whyte
Verified customer
投稿 Nov 27 2012