Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (embryonic testis E92)
Specification
embryonic testis E92
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/500
Additional data
Additional Notes
This antibody has stronger expression when compare to ab80892, however the expression is also cytoplasmic instead of nuclear. I think it might be detecting a different protein.
Abcam response
Thank you for sharing this data with us. We have not previously tested this antibody on marmoset tissue and from this abreview; it would seem that ab109250 does not work on this species, under these conditions. Abcam would welcome feedback from other customers using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. 煜欽 林
Verified customer
投稿 Feb 08 2013