Anti-NRF1 抗体 [2F9] (ab55744)
Key features and details
- Mouse monoclonal [2F9] to NRF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-NRF1 antibody [2F9]
NRF1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2F9] to NRF1 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment (GST-tag) corresponding to Human NRF1 aa 201-285.
Sequence:TQAQLRAFIPEMLKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTE EQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQ
Database link: Q16656 -
ポジティブ・コントロール
- WB: Human skeletal muscle lysate. IHC-P: Human kidney tissue.
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特記事項
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4 -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
2F9 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55744の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
|
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. -
組織特異性
Ubiquitously expressed with strongest expression in skeletal muscle. -
配列類似性
Belongs to the NRF1/Ewg family. -
翻訳後修飾
Phosphorylation enhances DNA binding. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4899 Human
- Omim: 600879 Human
- SwissProt: Q16656 Human
- Unigene: 654363 Human
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別名
- alpha pal antibody
- alpha palindromic binding protein antibody
- Alpha palindromic-binding protein antibody
see all
画像
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Anti-NRF1 antibody [2F9] (ab55744) at 1 µg/ml + Human skeletal muscle lysate
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Predicted band size: 54 kDaThis image was generated using the ascites version of the product.
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NRF1 antibody (ab55744) used in immunohistochemistry at µg/ml on formalin fixed and paraffin embedded human kidney.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (30)
ab55744 は 30 報の論文で使用されています。
- Chattopadhyay M et al. The portrait of liver cancer is shaped by mitochondrial genetics. Cell Rep 38:110254 (2022). PubMed: 35045282
- Asimi V et al. Hijacking of transcriptional condensates by endogenous retroviruses. Nat Genet 54:1238-1247 (2022). PubMed: 35864192
- Vangrieken P et al. Hypoxia-induced mitochondrial abnormalities in cells of the placenta. PLoS One 16:e0245155 (2021). PubMed: 33434211
- Rovito D et al. Myod1 and GR coordinate myofiber-specific transcriptional enhancers. Nucleic Acids Res 49:4472-4492 (2021). PubMed: 33836079
- Sönmezer C et al. Molecular Co-occupancy Identifies Transcription Factor Binding Cooperativity In Vivo. Mol Cell 81:255-267.e6 (2021). PubMed: 33290745