Anti-NOX1 抗体 (ab121009)
Key features and details
- Goat polyclonal to NOX1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NOX1 antibody
NOX1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NOX1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide:
DKATDIVTGLKQK
with a Cysteine residue linker, corresponding to internal sequence amino acids 490-502 of Human NOX1 (NP_008983.2) or 441-453 of Human NOX1 (NP_039249.1). -
ポジティブ・コントロール
- WB: HeLa, CaCo-2, A549 and U937 cell lysates; IHC-P: Human kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab121009 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121009の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
1 hour primary incubation is recommended for this product. |
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IHC-P |
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. -
組織特異性
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. -
配列類似性
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
細胞内局在
Cell projection > invadopodium membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 492016 Dog
- Entrez Gene: 27035 Human
- Entrez Gene: 701376 Rhesus monkey
- Omim: 300225 Human
- SwissProt: Q9Y5S8 Human
- Unigene: 592227 Human
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別名
- GP91 2 antibody
- Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
- Mitogenic oxidase 1 antibody
see all
画像
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All lanes : Anti-NOX1 antibody (ab121009) at 0.5 µg/ml
Lane 1 : CaCo-2 cell lysate
Lane 2 : CaCo-2 + peptide cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HeLa + peptide cell lysate
Lane 5 : A549 cell lysate
Lane 6 : A549 + peptide cell lysate
Lane 7 : U937 cell lysate
Lane 8 : U937 + peptide cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 65 kDaLysates in RIPA buffer. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling NOX1 with ab121009 at 7.5 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. An HRP-conjugated secondary antibody was used. Counterstained with hematoxylin.
Inset: Negative control showing staining of paraffin embedded human kidney, with no primary antibody.
データシートおよび資料
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Datasheet download
参考文献 (8)
ab121009 は 8 報の論文で使用されています。
- Jing Cao et al. Peroxidasin promotes diabetic vascular endothelial dysfunction induced by advanced glycation end products via NOX2/HOCl/Akt/eNOS pathway. Redox Biol 45:102031 (2021). PubMed: 34116361
- Wang Q et al. A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK-2 cells via regulating NADPH/oxidase-TGF-β/Smad signaling pathway. Phytother Res 35:5227-5240 (2021). PubMed: 34236110
- Zhang W et al. GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis. Aging (Albany NY) 13:22588-22610 (2021). PubMed: 34584017
- Yajun W et al. Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone. Front Pharmacol 12:723988 (2021). PubMed: 34658862
- Jung HY et al. NOX1 Inhibition Attenuates Kidney Ischemia-Reperfusion Injury via Inhibition of ROS-Mediated ERK Signaling. Int J Mol Sci 21:N/A (2020). PubMed: 32967113
- De Bessa TC et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis 10:143 (2019). PubMed: 30760703
- Alborzinia H et al. Golgi stress mediates redox imbalance and ferroptosis in human cells. Commun Biol 1:210 (2018). PubMed: 30511023
- Chocry M et al. Reversion of resistance to oxaliplatin by inhibition of p38 MAPK in colorectal cancer cell lines: involvement of the calpain / Nox1 pathway. Oncotarget 8:103710-103730 (2017). PubMed: 29262595