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RabMAb

Anti-NMDAR1 抗体 [EPR2480Y] (ab68144)

製品の概要

  • 製品名
    Anti-NMDAR1 antibody [EPR2480Y]
    NMDAR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2480Y] to NMDAR1
  • 由来種
    Rabbit
  • 特異性
    This antibody was raised against a phosphorylated peptide however the antibody recognises phosphorylated and non-phosphorylated protein.
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human NMDAR1 (phospho S889). The exact sequence is proprietary.
    (Peptide available as ab168728)

  • ポジティブ・コントロール
    • Mouse brain lysate; human brain tissue
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR2480Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab68144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).Can be blocked with NMDAR1 peptide (ab168728).
IP 1/30.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
    • 配列類似性
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
    • 翻訳後修飾
      NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
    • 細胞内局在
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    画像

    • All lanes : Anti-NMDAR1 antibody [EPR2480Y] (ab68144) at 1/1000 dilution

      Lane 1 : Human fetal brain cell lysate
      Lane 2 : Human forebrain cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 105 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)


      Exposure time: 30 seconds


      Blocking/diluting buffer: 5% NFDM/TBST

    • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68144 at a dilution of 1/100.

    参考文献

    This product has been referenced in:
    • Block A  et al. The GABAAa5-selective Modulator, RO4938581, Rescues Protein Anomalies in the Ts65Dn Mouse Model of Down Syndrome. Neuroscience 372:192-212 (2018). Read more (PubMed: 29292072) »
    • Yu X  et al. Endophilin A1 mediates seizure activity via regulation of AMPARs in a PTZ-kindled epileptic mouse model. Exp Neurol 304:41-57 (2018). WB . Read more (PubMed: 29481784) »

    See all 3 Publications for this product

    レビューと Q&A

    The peptide datasheet is at this link: https://www.abcam.com/index.html?datasheet=168728.

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
    Would you be so kind to check the batch number (GR257227-5) again since our system does not recognzie it.
    After reading through t...

    Read More

    Gracias por ponerse en contacto con nosotros. Dependiendo de la técnica que vaya a utilizar, le puede convenir mas usar un tipo de anticuerpos u otro. Ya que los anticuerpos policlonales son capaces de reconocer múltiples epítopos, éstos presentan ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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