Application
ChIP
Sample
Mouse Cell lysate - whole cell (bone marrow-derived macrophage)
Negative control
Untreated (un) BMDM cells. As shown in the graph provided.
Specification
bone marrow-derived macrophage
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
Santa Cruz Biotechnology antibody sc372 assayed in parallel (left bars, Abcam on the right bars):
Detection of the proximal TNF alpha promoter in bone marrow-derived macrophages (BMDMs) treated with 100 ng/ml of lipopolysaccaride (LPS).
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%Triton, 2mMEDTA, 20mMTrisCl, 0'15MNaCl
Additional data
Additional Notes
We used two ug of antibody per immunoprecipitation together with 20 to 50 ug of chromatin
Lucia Olalla from ABCAM already told us that the discount for the ABREVIEW will be appylied to our next order and the discount code (ABTRIAL-EVTBL)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 14 2016