NF-kB Signaling Pathway 抗体 Sampler Panel (ab228529)
製品の概要
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製品名
NF-kB Signaling Pathway Antibody Sampler Panel -
製品の概要
ab228529, NF-kB Signaling Pathway Antibody Panel, is a collection of 1 anti-rabbit (HRP) secondary antibody and 5 recombinant rabbit monoclonal antibodies against IKB alpha (p S36), IKB alpha, NFkB p105, NF-kB p65 (p S536), and NF-kB p65 provided in 10 µl trial sizes.
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特記事項
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols.
関連製品
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Related Products
- Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] (ab190205)
- Alexa Fluor® 647 Anti-NF-kB p65 antibody [E379] (ab190589)
- Alexa Fluor® 488 Anti-IKB alpha antibody [E130] (ab202644)
- Alexa Fluor® 647 Anti-IKB alpha antibody [E130] (ab202647)
- Alexa Fluor® 594 Anti-IKB alpha antibody [E130] (ab206346)
- Alexa Fluor® 555 Anti-IKB alpha antibody [E130] (ab206578)
- Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)
- Alexa Fluor® 594 Anti-NF-kB p65 antibody [E379] (ab214846)
- Anti-IKB alpha antibody [E130] - BSA and Azide free (ab215972)
- Anti-NFkB p105 / p50 antibody [E381] - BSA and Azide free (ab220803)
- APC Anti-IKB alpha antibody [E130] (ab224948)
- PE Anti-IKB alpha antibody [E130] (ab224949)
- Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
- Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] - BSA and Azide free (ab240059)
画像
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ICC/IF image of unpurified ab32518 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32518, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Predicted band: 105 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: NFkB p105 / p50 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-NFkB p105 / p50 antibody [E381] (ab32360) observed at 120, 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32360 was shown to specifically react with NFkB p105 / p50 when NFkB p105 / p50 knockout samples were used. Wild-type and NFkB p105 / p50 knockout samples were subjected to SDS-PAGE. ab32360 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of colon sections from mice, staining NF-kB p65 with ab32536.
Antigen retrieval was performed by microwave heating in citrate buffer, pH 6. Sections were incubated overnight with primary antibody (1/250) and staining was detected using ab80437 EXPOSE Rabbit specific HRP/DAB detection IHC kit. -
Primary - Anti-IKB alpha (phospho S36) antibody [EPR6235(2)] (ab133462) at 1/10000 dilution
Lane 1: HeLa cell lysate, 10 µg
Lane 2: HeLa cell lysate treated with FBS and Calyculin A, 10 µg
Secondary - HRP labeled goat anti-rabbit IgG, 1/2000 dilution
Predicted band: 35 kDa
Actual band: 40 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab228529 は 1 報の論文で使用されています。
- Del Favero G et al. Mycotoxin Altertoxin II Induces Lipid Peroxidation Connecting Mitochondrial Stress Response to NF-?B Inhibition in THP-1 Macrophages. Chem Res Toxicol 33:492-504 (2020). PubMed: 32022557