Anti-NDUFA4L2 抗体 (ab74138)
Key features and details
- Rabbit polyclonal to NDUFA4L2
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NDUFA4L2 antibody -
製品の詳細
Rabbit polyclonal to NDUFA4L2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide derived from an internal sequence of human NDUFA4L2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74138の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa).
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ELISA |
Use at an assay dependent concentration.
|
特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa). |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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配列類似性
Belongs to the complex I NDUFA4 subunit family. - Information by UniProt
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参照データベース
- Entrez Gene: 56901 Human
- Entrez Gene: 407790 Mouse
- SwissProt: Q9NRX3 Human
- SwissProt: Q4FZG9 Mouse
- Unigene: 75069 Human
- Unigene: 336856 Mouse
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別名
- FLJ26118 antibody
- NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 like 2 antibody
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2 antibody
see all
画像
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All lanes : Anti-NDUFA4L2 antibody (ab74138) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from COLO cells
Lane 3 : Extracts from HUVEC cells
Lane 4 : Extracts from HepG2 cells
Lane 5 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 10 kDa
Observed band size: <19 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab74138 は 4 報の論文で使用されています。
- Zheng J et al. Induction of the mitochondrial NDUFA4L2 protein by HIF-1a regulates heart regeneration by promoting the survival of cardiac stem cell. Biochem Biophys Res Commun N/A:N/A (2018). WB ; Rat . PubMed: 29953852
- Tahrir FG et al. Dysregulation of mitochondrial bioenergetics and quality control by HIV-1 Tat in cardiomyocytes. J Cell Physiol 233:748-758 (2018). PubMed: 28493473
- Miller BA et al. TRPM2 channels protect against cardiac ischemia-reperfusion injury: role of mitochondria. J Biol Chem 289:7615-29 (2014). WB ; Mouse . PubMed: 24492610
- Chen SJ et al. A splice variant of the human ion channel TRPM2 modulates neuroblastoma tumor growth through hypoxia-inducible factor (HIF)-1/2a. J Biol Chem 289:36284-302 (2014). WB . PubMed: 25391657