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RabMAb

Anti-NAK/TBK1 抗体 [EPR2867(2)-19] (ab109735)

製品の概要

  • 製品名
    Anti-NAK/TBK1 antibody [EPR2867(2)-19]
    NAK/TBK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2867(2)-19] to NAK/TBK1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human NAK/TBK1 aa 150-250 (phospho S172). The exact sequence is proprietary.
    Database link: Q9UHD2

  • ポジティブ・コントロール
    • HeLa, U937, HepG2, and 293T cell lysates, Human testis tissue, HeLa cells
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

For unpurified use at 1/1000 - 1/2000.

IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/400.

  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
    • 組織特異性
      Ubiquitous with higher expression in testis.
    • 配列類似性
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC 2.7.11.1 antibody
      • FLJ11330 antibody
      • FTDALS4 antibody
      • NAK antibody
      • NF kappa B activating kinase antibody
      • NF kB activating kinase antibody
      • NF-kappa-B-activating kinase antibody
      • Serine/threonine protein kinase TBK 1 antibody
      • Serine/threonine protein kinase TBK1 antibody
      • Serine/threonine-protein kinase TBK1 antibody
      • T2K antibody
      • TANK binding kinase 1 antibody
      • TANK-binding kinase 1 antibody
      • TBK 1 antibody
      • Tbk1 antibody
      • TBK1_HUMAN antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: NAK/TBK1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109735 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109735 was shown to recognize NAK/TBK1 when NAK/TBK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and NAK/TBK1 knockout samples were subjected to SDS-PAGE. ab109735 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded human testis tissue using ab109735 at a dilution of 1/250.

    • ab109735 staining NAK/TBK1 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/800). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

    • All lanes : Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 84 kDa

    参考文献

    This product has been referenced in:
    • Luo WW  et al. iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA. PLoS Pathog 13:e1006693 (2017). Read more (PubMed: 29155878) »
    • He X  et al. ERRa negatively regulates type I interferon induction by inhibiting TBK1-IRF3 interaction. PLoS Pathog 13:e1006347 (2017). WB ; Human . Read more (PubMed: 28591144) »

    See all 4 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (12% page)
    Sample
    Human Cell lysate - whole cell (epithelioid pancreatic carcinoma - panc-1 atcc cel)
    Specification
    epithelioid pancreatic carcinoma - panc-1 atcc cel
    Treatment
    mock transfected - empty vector
    Blocking step
    Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

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    投稿 Jan 17 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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