Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Trabecular Meshwork)
Permeabilization
No
Specification
Trabecular Meshwork
Blocking step
PBS superblock blocking buffer (Thermo Scientific, Prod # 37517) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS superblock buffer
Dilution
1/50
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Goat anti-Mouse IgM Cross Adsorbed Secondary Antib
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 21 2015