Application
IHC - Wholemount
Sample
Hydra vulgaris Tissue (morphogenesis)
Specification
morphogenesis
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA (Blocking solution)
Dilution
1/400
Secondary antibody
Dilution
1/750
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Animals were relaxed in 2% urtethane for 1 min and fixed overnight in 4 % PFA (PBS), pH 7.4 at 4°C.
Polyps were washed and permeabilised 3 x 20 min in 1x PBT (1x PBS containing 0.25 % TritonX-100). Animals were incubated in primary antibody diluted in 2% BSA/PBT over night at 4 °C.
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in Hydra vulgaris, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in Hydra vulgaris which will help us assess how well the antibody works in this species.
PubMed ID
28411398: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Oliver Holz
Verified customer
投稿 Jul 21 2017