Application
Western blot
Sample
Mouse Tissue lysate - other (Heart)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
20 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 19 kDa
Additional data
Additional Notes
line 1: Heart whole tissue lysate sample
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 07 2012