Application
Immunocytochemistry
Sample
Rat Cultured Cells (Subventricular zone neurospheres)
Permeabilization
No
Specification
Subventricular zone neurospheres
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: ,1% Triton X-100 and BSA 0,3% in PBS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
As you may see in the image the antibody is not selective for PLP since there is an almost perfect colocalization with Tuj1-positive neurons
Abcam response
Our Scientific Support team has been in contact with this customer, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 14 2015