製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Myelin PLP
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Goat, Cat, Human, Xenopus laevis, Common marmoset
    交差が予測される動物種: Rabbit, Chicken, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Myelin PLP.

    Immunogen の所有権に関して

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Rat Brain and Rat Spinal Cord tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab105784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/2000.

ターゲット情報

  • 機能
    This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
  • 関連疾患
    Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
    Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy.
  • 配列類似性
    Belongs to the myelin proteolipid protein family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HLD1 antibody
    • Lipophilin antibody
    • Major myelin proteolipid protein antibody
    • MMPL antibody
    • Myelin proteolipid protein antibody
    • MYPR_HUMAN antibody
    • PLP 1 antibody
    • PLP antibody
    • PLP/DM20 antibody
    • PLP1 antibody
    • PMD antibody
    • Proteolipid protein 1 antibody
    • SPG2 antibody
    see all

画像

  • All lanes : Anti-Myelin PLP antibody (ab105784) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • IHC-Fr image of myelin PLP staining on Mouse sciatic nerve sections using ab105784 (1:3000). Fresh frozen sections (10μM) and dried in fumehood for 2 hours before fixing with 10% formalin for 10 min. Sections were permeablized in 0.1% Triton X-100 in TBS for 10min. Sections were then blocked using 1% BSA for 10 mins at 21°C. ab105784 was diluted 1:2000 in TBS buffer (containing BSA) and sections were then incubated with ab105784 for 16 hours at 21°C. The secondary antibody used was Alexa Fluor® 594 conjugated Goat polyclonal to Rabbit IgG (1:1000).

    See Abreview

  • ab105784 staining rat adult brain sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/1000 in TBS/BSA/azide for 1h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • ab105784 staining human adult brainstem sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/2000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • ab105784 staining goat cerebellum sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/1000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • ab105784 staining mouse adult brain sections (grey matter, frontal cortex sagittal plane) by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab105784 at 1/750 in TBS/BSA/azide for 1h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.)

    See Abreview

参考文献

This product has been referenced in:
  • Dheer A  et al. Temporal and Spatial Changes in Glial Cells During Chronic Hypobaric Hypoxia: Role in Neurodegeneration. Neuroscience 383:235-246 (2018). Read more (PubMed: 29751055) »
  • Montagne A  et al. Pericyte degeneration causes white matter dysfunction in the mouse central nervous system. Nat Med 24:326-337 (2018). Read more (PubMed: 29400711) »
See all 4 Publications for this product

レビューと Q&A

1-10 of 17 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Rat Cultured Cells (Subventricular zone neurospheres)
Permeabilization
No
Specification
Subventricular zone neurospheres
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jul 14 2015

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (Sciatic nerve)
Specification
Sciatic nerve
Permeabilization
Yes - 0.1% TRITON X-100 in TBS
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

投稿 May 31 2013

Question
Answer

Thank you for your phone call.

DISCOUNT CODE: xxx for ab66043
DISCOUNT CODE: xxx for ab105784
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab66043 and ab105784 in dog. Each code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for dogand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your reply and I am sorry that we were not able to resolve the issue that you were having with our antibodies.As requested, I have credited back the cost of the original purchase.

Your credit note ID is *

I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thank you for your reply and for providing that information.

Both the ab29486 and ab105784 are giving the same staining pattern which is strange and so I was wandering if you had used a secondary only control on these samples. I am just curious to see if the secondary antibody itself could be giving some of this non-specific background. Also, according to our notes ab28486 will recognize both a 26 & 30kDa band, so I do believe that the intense band signal that is being detected at 26kDa is correct.

Some other protocol variations that might help reduce background are to reduce the primary antibody concentration to 1/500 and also further reduce the total amount of protein loaded into each well. I

If none of the suggestions above prove to be successful, then the we can look into either another replacement or a refund.

I do appreciate your patience in this matter while we try and sort this one out.

I look forward to your reply.

Read More

Answer

Thank you for your reply.

Hopefully the new antibody will help clear up the number of bands that you are seeing. You should receive the antibody tomorrow morning by 10.30am and your new order number (for tracking purposes only) is **********.

If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for your reply.

As you are not getting the correct signal in western blot using the antibody against rat samples, then I am happy to send you a replacement antibody free of charge.

The only other antibody in our catalogue against Myelin PLP that is at least predicted to work against canine tissue is ab28486:

https://www.abcam.com/Myelin-PLP-antibody-ab28486.html



If you are happy with this antibody as a replacement, then please let me know.

I look forward to your reply.

Read More

Answer

Thank you for that information.

I am sending you ab29475 to use as a positive control. Your order number is *******.

Please let me know how the positive control works out and if there is anything else I can help you with.

Read More

Question
Answer

Thank you for calling Abcam.

I have not been able to determine why you are getting a signal at 25kDa. As we had not tested this antibody on canine tissue, we were not sure how it would react, so there is a very good chance that the band is the correct target, just that it is slightly smaller than expected.

I was wondering if you were able to use rat brain lysate as a positive control, since that is what we recommended on the datasheet. If you do not have any rat brain lysate, then please let me know and I will be happy to send some (if you do wish for me to send some, please provide the original Abcam order number).

Please let me know if there is anything else I can help you with.

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Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (optic nerve)
Specification
optic nerve
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 03 2012

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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