Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-chicken HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 47 kDa Non-specific: 55 kb kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
WT mice
Positive control
Samples from mice with a myc-tagged gene
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 16 2014