SureLight® APC Anti-Myc tag 抗体 [9E10] (ab72580)
Key features and details
- SureLight® APC Mouse monoclonal [9E10] to Myc tag
- Suitable for: Flow Cyt, FRET
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
SureLight® APC Anti-Myc tag antibody [9E10]
Myc tag 一次抗体 製品一覧 -
製品の詳細
SureLight® APC Mouse monoclonal [9E10] to Myc tag -
由来種
Mouse -
標識
SureLight® APC. Ex: 652nm, Em: 657nm -
特異性
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
アプリケーション
適用あり: Flow Cyt, FRETmore details -
免疫原
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
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エピトープ
Epitope located at aa 410-419; EQKLISEEDL -
特記事項
Excitation max. 652 nm. Emission max. 657.5 nm. F/P 1:1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 8.20
Preservative: 0.013% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.1% BSA, 0.87% Sodium chloride, 97.37% Tris buffered saline -
Concentration information loading...
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特記事項(精製)
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used. -
ポリ/モノ
モノクローナル -
クローン名
9E10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
- DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
- DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
- DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
- FITC Anti-Myc tag antibody [9E10] (ab117599)
- Biotin Anti-Myc tag antibody [9E10] - C-terminal (ab197139)
- Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
- Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
- APC Anti-Myc tag antibody [9E10] (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] (ab32)
- HRP Anti-Myc tag antibody [9E10] (ab62928)
- PE Anti-Myc tag antibody [9E10] (ab72468)
- Biotin Anti-Myc tag antibody [9E10] (ab81658)
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Assay kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72580の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab73711 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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FRET |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. ab73711 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
FRET
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
細胞内局在
Nuclear -
別名
- c-myc tag antibody
- Myc Epitope Tag antibody
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab72580 は 2 報の論文で使用されています。
- Hörnström D et al. Molecular optimization of autotransporter-based tyrosinase surface display. Biochim Biophys Acta Biomembr 1861:486-494 (2019). PubMed: 30521785
- Gustavsson M et al. Surface expression of ?-transaminase in Escherichia coli. Appl Environ Microbiol 80:2293-8 (2014). PubMed: 24487538