Application
Western blot
Sample
Mouse Cell lysate - whole cell (B-cells)
Loading amount
30 µg
Specification
B-cells
Treatment
BL19LP overexpress MyD88
Gel Running Conditions
Reduced Denaturing (10% SDS-Acrylamide gel 150V, 1h)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/300
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T, 2.5% milk
Secondary antibody
Name
Non-Abcam antibody was used: goat-anti-mouse-IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
13 second(s)
Bands
Specific: -- kDa Non-specific: 23, 24, 48, 50, 60, 65 kDa
Positive control
Cells overexpressing MyD88 - verified by reporter positivity in FACS
Negative control
mouse fibroblasts
Additional data
Additional Notes
The antibody does not function with mouse MyD88
Abcam response
At the time of this Abreview we had not as yet tested this species. Thank you so much for testing this antibody in mouse.
We appreciate very much your efforts and are sorry to hear that the antibody did not work in this species/application.
We appreciate very much your efforts and are sorry to hear that the antibody did not work in this species/application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 08 2013