Application
Western blot
Sample
Pig Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS Tween 0,5% Lait 5% NaN3
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Clarity
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 24, 58, 70, 80, 150, 170, kDa
Additional data
Additional Notes
Lane 1: Ladder
Lane 2: mouse intestinal tissue
Lane 3: pig intestinal tissue
Have to try with a lower concentration of primary antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 08 2016