Mouse BAFF ELISA Kit (TNFSF13B) (ab119580)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse BAFF ELISA Kit (TNFSF13B)
BAFF キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 16 82pg/ml 6.47 = 7.9% 2 16 287pg/ml 15.52 = 5.3% 3 16 1221pg/ml 67.15 = 5.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 24 76pg/ml 7.14 = 9.4% 2 24 287pg/ml 16.07 = 5.6% 3 24 1209pg/ml 72.54 = 6% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
46.9 pg/ml - 3000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s mouse BAFF (TNFSF13B) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse BAFF (TNFSF13B) in cell culture supernatants, serum and plasma (heparin, EDTA).
A BAFF (TNFSF13B) specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BAFF (TNFSF13B) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse BAFF (TNFSF13B) amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-mouse BAFF antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- mouse BAFF antibody 1 x 100µl 1 x 100µl Lyophilized recombinant mouse BAFF standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究分野
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機能
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
組織特異性
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- ApoL related ligand TALL 1
- B cell Activating Factor
- B lymphocyte stimulator
see all -
参照データベース
- Entrez Gene: 24099 Mouse
- SwissProt: Q9WU72 Mouse
- Unigene: 28835 Mouse
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab119580 は 3 報の論文で使用されています。
- Werner A et al. Targeting B cells in the pre-phase of systemic autoimmunity globally interferes with autoimmune pathology. iScience 24:103076 (2021). PubMed: 34585117
- Yarchoan M et al. Effects of B cell-activating factor on tumor immunity. JCI Insight 5:N/A (2020). PubMed: 32434989
- Häsler J et al. VNAR single-domain antibodies specific for BAFF inhibit B cell development by molecular mimicry. Mol Immunol 75:28-37 (2016). PubMed: 27213814