Mouse Angiogenesis 抗体 Array - Membrane (24 Targets) (ab139697)
製品の概要
-
製品名
Mouse Angiogenesis Antibody Array - Membrane (24 Targets)
Angiogenesis Antibody Array antibody arrays 製品一覧 -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Semi-quantitative -
種交差性
交差種: Mouse -
製品の概要
ab139697 is for simultaneous detection of 24 Mouse Angiogenic factors. Suitable for all sample types.
Targets: Eotaxin, Fas Ligand, bFGF, G-CSF, GM-CSF, IFN-gamma, IGF-II, IL-1 alpha, IL-1 beta, IL-12 p40/p70, IL-12 p70, IL-13, IL-6, IL-9, Leptin, MCP-1, M-CSF, MIG, PF-4, TIMP-1, TIMP-2, TNF-alpha, Thrombopoietin.
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
-
特記事項
A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Multiplex Protein Detectionmore details
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Angiogenesis Antibody Array Membranes 4 units 8 units Biotin-Conjugated Anti-Cytokines 2 vials 4 vials Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab139697の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Multiplex Protein Detection |
Use at an assay dependent concentration.
|
特記事項 |
---|
Multiplex Protein Detection
Use at an assay dependent concentration. |
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab139697 は 3 報の論文で使用されています。
- Lee J et al. Melanoma topology reveals a stem-like phenotype that promotes angiogenesis. Sci Adv 3:e1701350 (2017). PubMed: 29075670
- Hedhli J et al. Multimodal Assessment of Mesenchymal Stem Cell Therapy for Diabetic Vascular Complications. Theranostics 7:3876-3888 (2017). PubMed: 29109784
- Khabbazi S et al. Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro. Sci Rep 6:31572 (2016). PubMed: 27514308