The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 59 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B antibody (ab118865)
ab118865 at 5 µg/ml staining Monoamine Oxidase B in Paraffin-embedded Human brain cortex tissue by immunohistochemistry. After incubation with ab118865 slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-Monoamine Oxidase B antibody (ab118865)
All lanes : Anti-Monoamine Oxidase B antibody (ab118865) at 1 µg/ml
Lane 1 : Human brain lysate without blocking peptide Lane 2 : Human brain lysate with blocking peptide