Anti-Mitofusin 1 抗体 - N-terminal (ab191853)
Key features and details
- Rabbit polyclonal to Mitofusin 1 - N-terminal
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Mitofusin 1 antibody - N-terminal
Mitofusin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mitofusin 1 - N-terminal -
由来種
Rabbit -
特異性
ab191853 is predicted to not cross-react with Mitofusin 2. -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Mitofusin 1 (N terminal). The exact sequence is proprietary. Peptide corresponds to 17 amino acids near the amino terminus (NP_284941).
Database link: Q8IWA4 -
ポジティブ・コントロール
- A431 cells and cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 84 kDa).
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特記事項 |
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ICC
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 84 kDa). |
ターゲット情報
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機能
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. -
組織特異性
Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. -
配列類似性
Belongs to the mitofusin family. -
翻訳後修飾
Ubiquitinated by MARCH5. -
細胞内局在
Cytoplasm and Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 55669 Human
- Omim: 608506 Human
- SwissProt: Q8IWA4 Human
- Unigene: 478383 Human
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別名
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
see all
画像
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Anti-Mitofusin 1 antibody - N-terminal (ab191853) at 1 µg/ml + A431 cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted? -
Immunocytochemistry analysis of A431 cells, labeling Mitofusin 1 using ab191853 at 5 μg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab191853 は 1 報の論文で使用されています。
- Kshirsagar S et al. A Combination Therapy of Urolithin A+EGCG Has Stronger Protective Effects than Single Drug Urolithin A in a Humanized Amyloid Beta Knockin Mice for Late-Onset Alzheimer's Disease. Cells 11:N/A (2022). PubMed: 36078067