Application
Immunocytochemistry
Sample
Human Cultured Cells (Human Melanoma cell line M238)
Permeabilization
No
Specification
Human Melanoma cell line M238
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Methanol
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 0.5% of BSA
Secondary antibody
Name
Non-Abcam antibody was used: BA-2001
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Host species: Mouse
Clonality: Monoclonal
Conjugation: Biotin
Additional data
Abcam response
We would like to thank the reviewer for submitting this Abreview for our product ab732. As the product had not yet been tested in ICC/IF before we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Rong Huang
Verified customer
投稿 Apr 16 2018