Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Embryo (Squashed polytene chromosomes dissected from 3rd i)
Specification
Squashed polytene chromosomes dissected from 3rd i
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA and 0.1% Triton 100 in PBS buffer
Dilution
1/200
Additional data
Additional Notes
MeCP2-transgenic fruit fly 3rd instar larvae raised at 25¯C was used. Polytene chromosomes were dissected and squashed for whole mount immunohistochemistry. Floating sample was co-stained with DNA marker DAPI.
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投稿 Dec 15 2014