Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat cerebral cortex section)
Specification
Rat cerebral cortex section
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA and 0.1% Triton 100 in TBS buffer
Dilution
1/500
Additional data
Additional Notes
10um rat cerebral cortex section was co-stained with MeCP2 and methylated histone H3K27Me3 antibodies. MeCP2 and H3K27Me3 showed co-localization in the nuclei of cortical neurons.
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投稿 Dec 11 2014