Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/5000
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Submittted image shows no Mast cell positivity.
However, smooth muscle and enterocyte cytoplasm are positive.
Abcam response
Many thanks for your valuable feedback. Abcam has not validated the combination of species/application used in this Abreview. It appears that under these experimental conditions, ab2378 does not specifically detect Mast Cell Tryptase in the Formalin/PFA-fixed paraffin-embedded sections of goat colon tissue. We welcome further customer feedback. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Aug 22 2014