Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Specification
Primary Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 5% PBS-T 0.05%
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you for your valuable feedback. Your data suggests that ab110258 may not be suitable to detect endogenous MTCO2 by Western blot in Rat Primary Neurons cell extracts under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 22 2014