Anti-MTCO2 抗体 [12C4F12] (ab110258)
Key features and details
- Mouse monoclonal [12C4F12] to MTCO2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-MTCO2 antibody [12C4F12]
MTCO2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [12C4F12] to MTCO2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- WB: Mitochondrial lysate from human heart tissue. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
ab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
12C4F12 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110258の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (11) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
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ICC/IF | (2) |
Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)
|
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
ICC/IF
Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal) |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. -
関連疾患
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
配列類似性
Belongs to the cytochrome c oxidase subunit 2 family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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別名
- COII antibody
- COX 2 antibody
- COX II antibody
see all
画像
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Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Mitochondrial lysate from human heart tissue at 5 µg
Predicted band size: 26 kDa -
Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.
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ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).
An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (219)
ab110258 は 219 報の論文で使用されています。
- Kiyokoba R et al. Mitochondrial dysfunction-induced high hCG associated with development of fetal growth restriction and pre-eclampsia with fetal growth restriction. Sci Rep 12:4056 (2022). PubMed: 35260712
- Ke PY et al. Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN. Cells 11:N/A (2022). PubMed: 35406696
- Saha B et al. Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5α in mitophagy. Cell Rep 39:110797 (2022). PubMed: 35545034
- Nývltová E et al. Coordination of metal center biogenesis in human cytochrome c oxidase. Nat Commun 13:3615 (2022). PubMed: 35750769
- Tan HWS et al. A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery. Nat Commun 13:3720 (2022). PubMed: 35764633