Anti-MT-ND1 抗体 (ab233289)
Key features and details
- Rabbit polyclonal to MT-ND1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-MT-ND1 antibody
MT-ND1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MT-ND1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human, Pig -
免疫原
Recombinant fragment corresponding to Human MT-ND1 aa 150-300. Two N-terminal tags, His-tag and GST-tag. Expressed in E.coli.
Database link: P03886 -
ポジティブ・コントロール
- IHC-P: Human liver tissue. WB: Recombinant human MT-ND1 protein; Pig liver tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. |
ターゲット情報
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機能
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
関連疾患
Defects in MT-ND1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
Defects in MT-ND1 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
Defects in MT-ND1 may be associated with susceptibility to Alzheimer disease mitochondrial (AD-MT) [MIM:502500]. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in MT-ND1 may be associated with non-insulin-dependent diabetes mellitus (NIDDM). -
配列類似性
Belongs to the complex I subunit 1 family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4535 Human
- Omim: 516000 Human
- SwissProt: P03886 Human
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別名
- Complex I, subunit ND1 antibody
- Mitochondrially encoded NADH dehydrogenase 1 antibody
- MT-ND1 antibody
see all
画像
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Anti-MT-ND1 antibody (ab233289) at 3 µg/ml + Pig liver tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Formalin-fixed, paraffin-embedded human liver tissue stained for MT-ND1 using ab233289 at 10 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for MT-ND1 using ab233289 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Anti-MT-ND1 antibody (ab233289) at 2 µg/ml + Recombinant human MT-ND1 protein
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab233289 は 1 報の論文で使用されています。
- Liu FF et al. Zinc Supplementation Forms Influenced Zinc Absorption and Accumulation in Piglets. Animals (Basel) 11:N/A (2020). PubMed: 33375418