Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rabbit Tissue lysate - whole (aorta)
Specification
aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Bands
Specific: 72 kDa
Exposure
30 second(s)
Positive control
loading control b actin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 24 2013