Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Diluent: Blocking solution without Trtion
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Native from an ectodermic tissue, olfactory stem cell could express MAP2 after spheres induction, as other members of mesenchymal stem cells family (Drela et al., 2013)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Antoine Veron
Verified customer
投稿 Nov 18 2016