製品名Anti-MAP1LC3A antibody [EP1528Y]
MAP1LC3A 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EP1528Y] to MAP1LC3A
アプリケーション適用あり: WB, IP, ICC, IHC-Pmore details
適用なし: Flow Cyt
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human MAP1LC3A (N terminal). The exact sequence is proprietary.
- Fetal brain cytoplasmic cell lysate Human brain tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
精製度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab52628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).|
|ICC||1/50 - 1/100.|
|IHC-P||Use at an assay dependent concentration.|
機能Probably involved in formation of autophagosomal vacuoles (autophagosomes).
組織特異性Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
配列類似性Belongs to the MAP1 LC3 family.
翻訳後修飾The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
細胞内局在Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
- Information by UniProt
- ATG8E antibody
- Autophagy-related protein LC3 A antibody
- Autophagy-related ubiquitin-like modifier LC3 A antibody
Anti-MAP1LC3A antibody [EP1528Y] (ab52628) at 1/50000 dilution + Fetal brain lysate at 10 µg
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Vishnyakova PA et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). Read more (PubMed: 28736722) »
- Cabang AB et al. Therapeutic effects of the euglenoid ichthyotoxin, euglenophycin, in colon cancer. Oncotarget 8:104347-104358 (2017). Read more (PubMed: 29262645) »