Anti-Liver Carboxylesterase 1/CES1 抗体 (ab115280)
Key features and details
- Goat polyclonal to Liver Carboxylesterase 1/CES1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Liver Carboxylesterase 1/CES1 antibody
Liver Carboxylesterase 1/CES1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Liver Carboxylesterase 1/CES1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Macaque monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Human Liver Carboxylesterase 1/CES1 aa 500-600 (internal sequence).
Database link: P23141 -
ポジティブ・コントロール
- Recombinant Human Liver Carboxylesterase 1/CES1 protein (ab114347) can be used as a positive control in WB. Human lung tissue. Human liver lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Constituents: 99% Tris buffered saline, 0.02% Sodium chloride, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115280の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 63 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. -
組織特異性
Expressed predominantly in liver with lower levels in heart and lung. -
配列類似性
Belongs to the type-B carboxylesterase/lipase family. -
翻訳後修飾
Contains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
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参照データベース
- Entrez Gene: 1066 Human
- Omim: 114835 Human
- SwissProt: P23141 Human
- Unigene: 558865 Human
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別名
- ACAT antibody
- Acyl coenzyme A cholesterol acyltransferase antibody
- Acyl-coenzyme A:cholesterol acyltransferase antibody
see all
画像
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Human Lung formalin-fixed, paraffin-embedded tissue stained with ab115280 at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-Liver Carboxylesterase 1/CES1 antibody (ab115280) at 0.03 µg/ml + Human Liver lysate in RIPA buffer (incubation time: 1 hour) at 35 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab115280 は論文での使用が確認できていません。