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RabMAb

Anti-Liver Carboxylesterase 1 抗体 [EP1375Y] (ab68190)

製品の概要

  • 製品名
    Anti-Liver Carboxylesterase 1 antibody [EP1375Y]
    Liver Carboxylesterase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1375Y] to Liver Carboxylesterase 1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Liver Carboxylesterase 1 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • Lysates from U937 cell line, human small intestine and human liver.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/25000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 62 kDa).
IP 1/30.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    • 機能
      Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
    • 組織特異性
      Expressed predominantly in liver with lower levels in heart and lung.
    • 配列類似性
      Belongs to the type-B carboxylesterase/lipase family.
    • 翻訳後修飾
      Contains sialic acid.
      Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.
    • 細胞内局在
      Endoplasmic reticulum lumen.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ACAT antibody
      • Acyl coenzyme A cholesterol acyltransferase antibody
      • Acyl-coenzyme A:cholesterol acyltransferase antibody
      • Brain carboxylesterase hBr1 antibody
      • Carboxyesterase ES-3 antibody
      • Carboxylesterase antibody
      • Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody
      • Carboxylesterase 1 antibody
      • Carboxylesterase 1 deficiency, included antibody
      • Carboxylesterase 2, formerly antibody
      • CE 1 antibody
      • CEH antibody
      • Ces-1 antibody
      • CES1 antibody
      • CES2 antibody
      • CESDD1 antibody
      • Cholesterol ester hydrolase, neutral, macrophage-derived antibody
      • Cholesteryl ester hydrolase antibody
      • Cocaine carboxylesterase antibody
      • EC 3.1.1.1 antibody
      • Egasyn antibody
      • ES-HTEL antibody
      • ES-x antibody
      • Es22 antibody
      • EST1_HUMAN antibody
      • Esterase 22 antibody
      • Esterase antibody
      • hCE 1 antibody
      • HMSE antibody
      • HMSE1 antibody
      • Liver carboxylesterase 1 antibody
      • Liver carboxylesterase 3 antibody
      • Methylumbelliferyl acetate deacetylase 1 antibody
      • MGC117365 antibody
      • MGC156521 antibody
      • Monocyte carboxylesterase deficiency, included antibody
      • Monocyte esterase deficiency, included antibody
      • Monocyte/macrophage serine esterase antibody
      • PCE-1 antibody
      • pI 5.5 esterase antibody
      • Proline-beta-naphthylamidase antibody
      • REH antibody
      • Retinyl ester hydrolase antibody
      • Serine esterase 1 antibody
      • Ses-1 antibody
      • SES1 antibody
      • TGH antibody
      • Triacylglycerol hydrolase antibody
      see all

    画像

    • All lanes : Anti-Liver Carboxylesterase 1 antibody [EP1375Y] (ab68190) at 1/50000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : human small intestine lysate
      Lane 3 : human liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 62 kDa
      Observed band size: 55 kDa (why is the actual band size different from the predicted?)

    • Ab68190 staining Liver Carboxylesterase 1 in U-937 (Human histiocytic lymphoma monocyte) by Flow Cytometry. Cells were fixed with 2% paraformaldehyde. The sample was incubated with primary antibody at 1:150 dilution (pink), A Goat anti-rabbit IgG (FITC) was used as a secondary antibody at 1:150 dilution. Rabbit monoclonal IgG was used as the isotype control (green).

    参考文献

    ab68190 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Retina Sections)
    Permeabilization
    Yes - Triton 100x
    Specification
    Retina Sections
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Wenjing Wu

    Verified customer

    投稿 Jun 27 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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