Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Derived Breast Cancer Cell Line)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
Mouse Derived Breast Cancer Cell Line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye Goat Anti-Rabbit 800 CW (Licor)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Infrared
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 70 kDa Non-specific: 24 kDa
Additional data
Additional Notes
The picture shows two blots (one used for testing the dilution 1:1000 and the other for the dilution 1:2000).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 09 2017